T4 DNA LIGASE

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1.What is the T4 DNA LIGASE ?

T4 DNA Ligase, also known as Escherichia coli T4 DNA Ligase, is an enzyme derived from the bacteriophage T4 that infects E. coli bacteria. It is a crucial component in molecular biology and genetic engineering, playing a significant role in the manipulation and analysis of DNA molecules. T4 DNA Ligase possesses the ability to catalyze the formation of phosphodiester bonds between neighboring 3′-hydroxyland 5′-phosphate ends in double-stranded DNA, as well as closing single-stranded nicks in double-stranded DNA. This enzyme is supplied with a 10x concentrated ligation buffer that includes ATP, which is essential for its function.

2.What is the CAS number for T4 DNA LIGASE ?

The CAS number of T4 DNA LIGASE is 9015-85-4.

3.What are another words for T4 DNA LIGASE ?

Synonyms?for?T4 DNA LIGASE 9015-85-4:ATP-dependentDNA ligase; CircLigase; DNA joinase; DNA ligase; DNA ligase I; DNA-joiningenzyme; Deoxyribonucleate ligase; Deoxyribonucleic acid joinase;Deoxyribonucleic acid ligase; Deoxyribonucleic joinase; Deoxyribonucleicligase; Deoxyribonucleic-joining enzyme; E.C. 6.5.1.1; NHR ligase; Nucleotideligase; Pfu DNA Ligase; Polynucleotide ligase; Polynucleotide synthetase;Polynucleotide synthetase (ATP); Sealase; Thermophage DNA ligase

4.What is T4 DNA LIGASE (9015-85-4) used for?

Forms an energy dependent phosphodiester linkage between the termini of adjacent polynucleotides of duplex DNA. For ligation of cloning vector and restriction insert fragments

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